primary antibodies raised against: proliferating cell nuclear antigen (pcna)  (Abnova)

Journal: Cells

Article Title: Progenitor Cells Activated by Platelet Lysate in Human Articular Cartilage as a Tool for Future Cartilage Engineering and Reparative Strategies

doi: 10.3390/cells9041052

Figure Lengend Snippet: Experimental design of cell cultures (articular chondrocytes (ACs) and chondro-progenitors (CPCs) from human articular cartilage biopsies. ( A ) Representative illustration of biopsy handling to obtain ACs culture and cartilage chip culture. ( B ) Optical images of cartilage chips after 15–20 days in culture with cells coming out to the medium supplemented with platelet lysate (PL) versus fetal bovine serum (FBS) and ( C ) representative immunohistological distribution of proliferating cell nuclear antigen (PCNA)-positive cells inside tissue in both culture conditions ( N = 3). ( D ) Histogram showing the percentage of PCNA-positive cells in cartilage chips maintained in culture with FBS or PL. Data are represented as mean ± SEM ( N = 3, * p < 0.05 versus ACs 10% FBS by Student’s t -test analysis). All scale bars correspond to 100 μm.

Article Snippet: After washes in PBS and incubation with 20% NGS for 1 h to inhibit nonspecific binding, the slices were incubated overnight at 4 °C with primary antibodies raised against: type II collagen, 1:250 diluted in 10% NGS (CIICI-Developmental Studies Hybridoma Bank, University of Iowa); type X collagen, 1:1000 diluted in 10% NGS (Abcam, Cambridge, UK); and proliferating cell nuclear antigen (PCNA), 1:200 diluted in 10% NGS (Abnova, Taipei City, Taiwan).

Techniques: